Nanoparticle-Membrane Interactions Studied with Lipid Bilayer Arrays

High-throughput formation of lipid bilayer membrane arrays with an asymmetric lipid composition

We present a micro-device in which more than 10,000 asymmetric lipid bilayer membranes are formed at a time on micro-chamber arrays. The arrayed asymmetric lipid bilayers, where lipid compositions are different between the inner and outer leaflets, are formed with high efficiency of over 97% by injecting several types of liquids into a micro-device that has hydrophilic-in-hydrophobic surfaces. ...

Nanoparticle–membrane interactions

Screening ion-channel ligand interactions with passive pumping in a microfluidic bilayer lipid membrane chip.

We describe a scalable artificial bilayer lipid membrane platform for rapid electrophysiological screening of ion channels and transporters. A passive pumping method is used to flow microliter volumes of ligand solution across a suspended bilayer within a microfluidic chip. Bilayers are stable at flow rates up to ∼0.5 μl/min. Phospholipid bilayers are formed across a photolithographically defin...

Imaging Lipid Microdomains in a Microfluidic Bilayer Lipid Membrane Chip

A microfluidic bilayer lipid membrane (BLM) system supporting correlated opto-electrical membrane characterization has been developed and applied to the imaging of phase-separated lipid microdomains by confocal microscopy. The device consists of a microfluidic network supporting rapid perfusion fabricated in a thermoplastic substrate, bonded to a thin PVDC partition and supported by a thin cove...

Vesicle fusion with bilayer lipid membrane controlled by electrostatic interaction

The fusion of proteoliposomes is a promising approach for incorporating membrane proteins in artificial lipid membranes. In this study, we employed an electrostatic interaction between vesicles and supported bilayer lipid membranes (s-BLMs) to control the fusion process. We combined large unilamellar vesicles (LUVs) containing anionic lipids, which we used instead of proteoliposomes, and s-BLMs...